TABLE 5

Complementation by SGS1 truncation alleles

top3 sgs1 slow-growth suppression
AlleleMMS hypersensitivityslx4 lethalityaHyper-recombinationLiquid growthFOA/-leu growthGal over-expressiontop1 sgs1 slow growth
sgs1::loxP
SGS1+++++++
sgs1-hd+ / −+ / −+ / −+/−
ΔN158HbNDHNDH
ΔN322ND+/−ND+
ΔN484NDNDND++
ΔN644ND+
ΔC200++++ / −+ / −++
ΔC300+/−ND
ΔC795+/−+ / −ND+/−ND+
  • a Strain NJY561 (sgs1 slx4) carrying plasmid pJM500 (SGS1/ URA3/ADE3) was transformed with the indicated SGS1 alleles in pRS415. The transformants were then streaked onto synthetic complete medium containing 1 mg/ml 5-FOA.

  • b Hypermorphic phenotype.

  • + , full complementation; −, no complementation; +/ −, intermediate complementation; ND, not determined.