TABLE 4

Intragenic complementation of sgs1 slx4 synthetic lethality

Complementing DNAVectorsgs1-hdsgs1C795sgs1C997sgs1C1247
SGS1++++++++++
Vector
sgs1N158++++++
sgs1N322++++++
sgs1N644++
  • Strain NJY561 (sgs1 slx4) carrying plasmid pJM500 (SGS1/ URA3/ADE3) was cotransformed with the indicated SGS1 alleles on LEU2 (rows) and TRP1 (columns) plasmids as in Figure 8. The transformants were then streaked onto synthetic complete medium containing 1 mg/ml 5-FOA. Wild-type growth on this medium is indicated by + + .