TABLE 3

Complementation of the sgs1 hyper-recombination phenotype

Percentage marker loss
Complementing DNAlys2::CAN1::LYS2RDN1::URA3::RDN1
SGS10.03 ± 0.01 (1.0×)1.23 ± 1.78 (1.0×)
Vector0.16 ± 0.03 (5.3×)11.6 ± 6.5 (9.4×)
sgs1-hd0.15 ± 0.04 (5.0×)11.5 ± 18.0 (9.3×)
sgs1N1580.49 ± 0.25 (16×)14.0 ± 7.6 (11.4×)
sgs1N158-hd0.27 ± 0.15 (9.0×)ND
sgs1N3220.17 ± 0.07 (5.6×)12.6 ± 7.7 (10.2×)
sgs1N6440.16 ± 0.04 (5.3×)13.6 ± 4.6 (11.1×)
sgs1C2000.04 ± 0.01 (1.3×)1.14 ± 1.34 (0.9 ×)
sgs1C3000.16 ± 0.04 (5.3×)10.0 ± 2.3 (8.1 ×)
sgs1C7950.05 ± 0.01 (1.7×)2.35 ± 1.40 (1.9 ×)
  • Strain NJY540 (sgs1::loxP) containing the URA3 and CAN1 genes integrated at RDN1 and LYS2, respectively (Keil and McWilliams 1993), was stably transformed with the indicated SGS1 alleles. After 3 days growth in the absence of selection, the percentage of cells that were canavanineR or ura was determined. The data are presented as the mean of seven independent colonies with the standard deviation and fold increase over wild type. ND, not determined.