TABLE 1

S. cerevisiae strains used in this study

StrainGenotypeReference or source
K1875MATa ade2-1 ura3-52 his4-260trp1-H3 leu2-3,112lys2ΔBX-CAN1-LYS2 canl rDNA::ADE2 rDNA::URA3Keil and McWilliams (1993)
W303-1aMATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100Thomas and Rothstein (1989)
AMR60W303-1α top3-2::HIS3 sgs1-3::TRP1Lu et al. (1996)
CHY125MATa ade2-1 ade3::hisG ura3-1 his3-12,15 trp1-1 leu2-3,112 can1-100Chris Hardy
NJY531CHY125 sgs1::loxPThis study
NJY540K1875 sgs1::loxPThis study
NJY561MATα ade2-1 ade3::hisG ura3-1 his3-12,15 trp1-1 leu2-3,112 can1-100 sgs1::loxP slx4::JoxP [pJM500::SGS1 URA3 ADE3]This study
NJY598MATa ade2-1 ade3::hisG ura3-1 his3-12,15 trp1-1 leu2-3,112 sgs1::loxP top1::loxPKAN-loxPThis study