TABLE 4

fru-expressing neurons in the male and female CNS of wild-type and fruitless mutants: total number of labeled neurons from both sides of the brain and ventral nerve cord

GenotypeBrainVentral nerve cord
Male
WT192.5 ± 22.5 (2)194.0 ± 9.5 (3)
fru1204.5 ± 7.5 (2)48.0 ± 6.0 (2)
fru2289.5 ± 46.5 (3)157.3 ± 0.3 (3)
fru3296.0 ± 62.0 (3)123.3 ± 38.4 (3)
fru4199.0 ± 42.0 (3)216.0 ± 58.0 (3)
frusat534.0 ± 35.9a (5)234.6 ± 27.7b (3)
Female
WT383.6 ± 23.5 (3)129.5 ± 9.1 (6)
fru1546.0 ± 51.9 (4)368.0 ± 54.0d (5)
fru2430.0 ± 5.8 (3)128.3 ± 18.9 (3)
fru3213.0 ± 45.5c (3)58.3 ± 9.9 (3)
fru4376.0 ± 39.0 (3)92.5 ± 39.5 (3)
frusat617.0 ± 46.1 (3)253.3 ± 14.4e (3)
  • Numbers ± SEMs are averages for neurons that were labeled with the S1 probe (see materials and methods) in CNS regions of Canton-S wild-type (WT) and fru-mutant males and females. The neuronal groups indicated are those described in the text (see also Figure 5, A and B). Neurons that are part of the three groups in the anterior protocerebrum (groups 3–5) were counted as a single group. In females, neuronal groups in the brain were counted, because the male-specific groups 7 and 9 are not present. For all genotypes except fru1 and frusat females, neurons were heavily labeled with the S1 probe and, thus, were distinguishable from neighboring unlabeled neurons. In fru1 and frusat females, many neurons were labeled heavily; a new class of moderately and lightly labeled neurons that were not found in animals of any other genotype were also detected. For this analysis, only the heavily labeled neurons were counted. From the statistical analyses, all comparisons of mean values were not significant, except as indicated (see footnotes).

  • a The average number of labeled neurons in the brains of frusat males is significantly different from the averages determined from scoring pharate-adult brains from males of all other genotypes tabulated.

  • b The average number of labeled neurons in the ventral nerve cord of frusat males is significantly different from the value determined by scoring the ventral nerve cord of fru1 males.

  • c The average number of labeled neurons in fru3 female brains is significantly different than those determined for fru1 and fru4 female brains.

  • d The average number of labeled neurons in the ventral nerve cord of fru1 females is significantly different from values for the ventral nerve cords of Canton-S, fru2, fru3, and fru4 females.

  • e The average number of labeled neurons in the ventral nerve cord of frusat females is significantly different from that determined for the ventral nerve cord of fru3 females.