TABLE 2

Germination on 200 mm NaCl medium of the F2 progeny of crosses involving sañ mutants and wild-type individuals

Cross (2 × s)Genetic background of the mutant strainF2 seedsPenetrance (%)χ2 (3:1)
SownGerminatedObservedExpected
Ws-2 × T78e (sañ1-1a)Ws-21982550.558.8 ± 16.10.69
Col × T78e (sañ1-1a)3608190.017.40
Col × N1a (sañ1-2)Col2014079.650.4 ± 24.18.60
Ler × N1a (sañ1-2)4157774.212.05
Col × N20a (sañ1-3)Col4397669.283.4 ± 4.82.94
Ler × N20a (sañ1-3)4157269.42.70
Ler × N39 (sañ1-4)Ler2084178.865.7 ± 16.51.33
Col × N39 (sañ1-4)28478109.923.90
Ler × E47a(sañ3-1)Ler2063975.769.6 ± 10.80.20
Col × E47a (sañ3-1)68514785.87.20
Col × N6a (sañ3-2)Col1863166.673.4 ± 10.40.21
Ler × N6a (sañ3-2)3635863.91.10
Col × N10a (sañ4-1a)Col1973979.284.8 ± 3.50.17
Ler × N10a (sañ4-1a)4679783.10.03
Col × N10e (sañ4-1b)Col2024181.176.6 ± 0.50.08
Ler × N10e (sañ4-1b)64512175.00.07
Ws-2 × T20 (sañ4-2)Ws-23126076.959.8 ± 6.14.05
Ler × T20 (sañ4-2)3034660.70.00
Col × T20 (sañ4-2)3056483.98.10
Col × N3c (sañ5)Col1072178.581.0 ± 9.20.00
Ler × N3c (sañ5)2072854.17.70
  • All mutant strains, which are noted according to their protocol numbers, were homozygous. Allele names indicated in parentheses were given after complementation analysis (see Table 4). ♀, pollen recipient; ♂, pollen donor. The χ2 values represent the fit of the data to an expected 3:1 (wild type:mutant) phenotypic segregation and were obtained after calculating the number of expected germinated seedlings, multiplying the number of seeds sown by 0.25 and by the penetrance of the mutant phenotype in its original genetic background at that NaCl concentration. Numbers in italics indicate that the null hypothesis tested was rejected.