TABLE 4

β-Galactosidase assays with Tyb::LacZ fusions

Units of β-galactosidasec
StrainaGenotypebNo plasmiddpMB38-9mer (WT)epMB38-9mer (fusion)e
JC3641.8 ± 0.323 ± 5243 ± 46
DG1520rad522.3 ± 0.914 ± 6184 ± 43
yAR291rad502.3 ± 0.425 ± 0.4172 ± 78
yAR306rad511.6 ± 0.123 ± 1220 ± 78
yAR292rad542.1 ± 0.217 ± 7324 ± 108
yAR294rad572.3 ± 0.315 ± 4173 ± 18
yAR348cdc93.5 ± 0.428 ± 5237 ± 55
  • a Strains are described in Table 1.

  • b Only relevant genotype is indicated.

  • c Units are nmol of ONPG converted/mg protein/minute.

  • d Average of three experiments.

  • e Average of two experiments.