Effects of expressing various gene products with the maternal GAL4 system

Relative viability of P{UAS-X} progeny (% female:% male)b
NGT femaleslacZ activityarunt.T (232)en.Y (4-1)dpp.S (42B.4)Pnt.P1 (3)
11129 ± 48100:10050:2080:100100:100
31307 ± 9190:1070:6060:50
40603 ± 7810:<10:0
11/11714 ± 30210:00:010:<5<5:0
31/31780 ± 10420:00:0<5:<50:0
40/401776 ± 2120:00:00:0
  • a β-Galactosidase activity is measured on staged embryos (7.25 hr) using the luminescent Galacto-Light Plus substrate (Tropix). The numbers reported are × 10−3 and represent the average and standard error of measurements done with portions (1/10) of lysates prepared from >10 single embryos.

  • b Relative viability was determined by counting the number of UAS carrying adult progeny relative to their siblings that carry a balancer (or other marked chromosome). The relative viability of P{UAS-runt.T}232 was measured relative to the second chromosome balancer CyO. The P{UAS} transposons for en, dpp, and pnt[P1] are all on the third chromosome. Viability crosses with these transgenes involved TM3 or ruPrica. The data shown are reported to the nearest decile, except for crosses that produced <5% of the expected number of viable UAS-X progeny. These data are all from crosses that yielded at least 50, and typically >200, sibling progeny.