Developmental time course of NGT-driven gene expression

Maternal genotypePaternal genotypeStage (hr)aβ-Galactosidase activity (× 10−3)b
NGT40/NGT40UAS-lacZ/UAS-lacZ3.5201 ± 66
NGT40/NGT40UAS-lacZ/UAS-lacZ4.5864 ± 237
NGT40/NGT40UAS-lacZ/UAS-lacZ5.51365 ± 181
NGT40/NGT40UAS-lacZ/UAS-lacZ7.251776 ± 212
NGT40/NGT40UAS-lacZ/UAS-lacZ8.51479 ± 120
NGT40/NGT40UAS-lacZ/UAS-lacZ10.51176 ± 455
UAS-lacZ/UAS-lacZNGT40/NGT407.252.1 ± 0.3
y w/y wUAS-lacZ/UAS-lacZ7.253.8 ± 0.4
  • a Embryos were selected at the onset of gastrulation based on appearance of the cephalic and ventral furrows. Embryos at this stage were defined as 3.5 hr old. Older embryos were obtained by aging them from the time of gastrulation.

  • b β-Galactosidase activity was measured on aliquots prepared from single staged embryos as described in materials and methods. The numbers shown indicate the relative light units ± SD for measurements made on at least five embryos from each stage.