TABLE 2

Nucleotide sites experiencing host-correlated reversions

SiteNo.GeneA. A.AS1SC1SCS1SCSC1S1SC2SCS2SCSC2
44201AD → GaCAAACCCC
47001A, A*N → KtGTTTGGGG
48051A, A*T → TaaGAAaaaa
3191CV → FgTGGGTTTT
5001DL → LgAGGGAAAA
9211JR → HggggggAGG
13056FG → DgAGAGAGAG
14601FQ → EcccGCcccc
20094FS → TtATTTATAT
20931FL → FcccTCcccc
21672FH → QtGTTTGTTT
  • Column 1 shows the site of substitution, column 2 the number of independent reversions occurring at that site, columns 3 and 4 the gene affected and the amino acid replacement, respectively. Column 5 (A) shows the nucleotide at this position in the ancestral genotype. Columns 6–9 and 10–13 show bases in the evolved isolates of replicates 1 and 2, respectively. Isolate S1 is depicted in both columns 6 and 10 since it is the starting isolate for both replicates. All evolved nucleotides are capitalized and nucleotide reversions to any previous nucleotide state are capitalized and underlined. Of the 20 total reversions occurring at the 11 different sites, 14 occurred in gene F, the major capsid gene.