TABLE 1

AFLP primers used for bulk segregant analysis

Source
This studyb
PrimeraGIBCO BRLcStandard AFLPcSequence-specific AFLP
EcoRI seriesAAC, AAG, ACA, ACC ACG, ACT, AGC, AGGAAA, AAT, AGA, AGT ATA, ATC, ATG, ATTGAA, GAC, GAG, GAT GTA, GTC, GTG, GTT
MseI seriesCAA, CAC, CAG, CAT CTA, CTC, CTG, CTTCCA, CCC, CCG, CCT CGA, CGC, CGG, CGTTCA, TCC, TCG, TCT TGA, TGC, TGG, TGT
  • a Designates the three-nucleotide sequence added to the 3′ end of the core sequence. The core sequence of EcoRI series is 5′-AGACTGCGTACCAATTC-3′ and the core sequence of MseI series is 5′-GATGAGTCCTGAG TAA-3′.

  • b Primers other than those supplied by GIBCO BRL were designed by the Wise lab and synthesized by the ISU DNA Synthesis and Sequencing Facility.

  • c Primers for both standard and sequence-specific AFLP.