TABLE 3

Phenotypes and sequence alterations of ced-3 mutants

GenotypeMutant/mutant: no. extra cells in anterior pharynx ± SDMutant/+ : no. extra cells in anterior pharynx ± SDNucleotide changeAmino acid/splice change
Wild type0.13 ± 0.4 (40)
sDf210.07 ± 0.3 (15)Large deletion
Recessive allelesa
n24240 (15)0.07 ± 0.3 (15)AGA → AATR55N
n29230 (15)0.13 ± 0.4 (15)GCG → GTGA347V
n24460.13 ± 0.4 (15)0.13 ± 0.4 (15)GTG → TTGV311L
n24490.2 ± 0.4 (15)0.13 ± 0.4 (15)CGC → CACR51H
n24250.3 ± 0.6 (17)0.13 ± 0.4 (15)GGC → GACG277D
n24470.8 ± 0.6 (15)0.07 ± 0.3 (15)TCG → TTGS446L
n30010.8 ± 1.0 (15)0.07 ± 0.3 (15)CGT→ TGTR242C
n24271.2 ± 0.9 (19)0 (15)GGA → AGAG474R
n24431.8 ± 1.7 (15)0.07 ± 0.3 (15)CCG→ TCGP400S
n24382.1 ± 1.2 (10)0.07 ± 0.3 (15)GGA → AGAG474R
n24366.2 ± 1.5 (10)0.13 ± 0.4 (15)CTT → TTTL269F
n28777.0 ± 1.5 (10)0.3 ± 0.6 (15)CTT → TTTL269F
n29217.9 ± 2.3 (10)0 (15)CCT → CTTP241L
n10408.3 ± 1.7 (31)0.13 ± 0.4 (15)CTC → TTCL27F
n28618.4 ± 1.6 (10)0 (15)GGT → AGTG261S
n11648.6 ± 1.4 (15)0.2 ± 0.4 (15)GCA → GTAA449V
n11298.7 ± 1.3 (10)0.07 ± 0.3 (15)GCA → GTAA449V
n24529.5 ± 1.5 (15)0 (15)deletion
n19499.5 ± 2.3 (10)0.3 ± 0.5 (15)CAA → TAAQ412ochre
n28859.6 ± 1.3 (10)0.07 ± 0.3 (15)GAG → AAGE456K
n28709.7 ± 1.6 (10)0.2 ± 0.4 (15)AGA → AAAR429K
n116310.2 ± 1.7 (15)0.13 ± 0.4 (15)TCC → TTCS486F
n299810.3 ± 1.7 (15)0.08 ± 0.3 (12)TGG→ TAGW436amber
n272210.6 ± 1.0 (15)0.2 ± 0.4 (15)GCA → GTAA418V
n292410.6 ± 1.7 (14)0.13 ± 0.4 (15)GAG → AAGE318K
n242910.6 ± 1.6 (15)0.2 ± 0.4 (15)TCA → TTAS314L
n288810.6 ± 1.7 (10)0 (15)CGA→ TGAR154opal
n244510.7 ± 1.1 (13)0.13 ± 0.4 (15)TAA → AAAochre504K
n285410.7 ± 1.8 (10)0.3 ± 0.5 (15)G|gattt → G|gttcgExon 5 donor
n242610.7 ± 2.1 (10)0.3 ± 0.6 (15)GAG → AAGE483K
n116510.9 ± 1.5 (15)0.13 ± 0.4 (15)CAG → TAGQ404amber
n128610.9 ± 1.5 (15)0.07 ± 0.3 (15)TGG → TGAW428opal
n244410.9 ± 1.9 (10)0.2 ± 0.4 (15)GCA → ACAA420T
n285910.9 ± 1.9 (10)0.3 ± 0.5 (15)TGG → TAGW406amber
n292210.9 ± 1.5 (10)0.07 ± 0.3 (15)GCA → ACAA420T
n244211.0 ± 1.3 (15)0.13 ± 0.4 (15)GGA → GAAG243E
n272111.1 ± 1.7 (15)0.07 ± 0.3 (15)CAC → TACH315Y
n288911.1 ± 1.2 (10)0.2 ± 0.4 (15)GAG → AAGE318K
n71711.2 ± 2.0 (10)0 (15)cag|CA → caa|CAExon 7 acceptor
n243911.3 ± 1.3 (15)0 (15)CTC → TTCL30F
n272011.6 ± 1.5 (15)0.2 ± 0.6 (15)CAC → TACH315Y
n271911.6 ± 1.9 (15)0.07 ± 0.3 (15)GGA → AGAG65R
n245411.6 ± 2.4 (15)0.07 ± 0.3 (15)GCT → ACTA466T
n243211.7 ± 1.2 (10)0.07 ± 0.3 (15)TAC → AACY235N
n283011.7 ± 1.5 (15)0.07 ± 0.3 (14)GGA → AGA, GGA → AGAG65R and G474R
n71811.8 ± 1.1 (10)0.07 ± 0.3 (15)CTC → TTCG65R
n300212.1 ± 1.5 (15)0 (15)GAG|gta → GAG|ataExon 7 donor
n288312.2 ± 1.1 (15)0.13 ± 0.4 (15)TCA → CCAS314P
Weakly semidominant allelesb
n244011.7 ± 1.7 (15)0.5 ± 0.6 (15)CGCAA → CGTAAQ401ochre/splice donor
n287111.7 ± 1.7 (15)1.0 ± 0.8 (15)CGA→ CAAR359Q
n243011.8 ± 1.2 (10)0.73 ± 0.6 (15)GCT → GTTA466V
n243312.4 ± 1.0 (10)0.5 ± 0.9 (15)GGC → AGCG360S
  • sDf21 is a deficiency spanning ced-3 (see materials and methods). Numbers in parentheses indicate the number of animals observed. Nucleotide change: altered residues are underlined, wild-type sequence is to the left of each arrow, and mutant sequence is to the right. Amino acid/splice change: numbers indicate the amino acid residue. For scoring mutant/mutant animals, the ced-3 alleles were separated from background mutations, except for some that were scored in the following genetic backgrounds: ced-3(n1163); egl-1(n487), ced-3(n1164); egl-1(n487), sem-4(n1378) ced-1(e1735); ced-11(n2744); ced-3(n2998), sem-4(n1379); ced-5(n2098) ced-3(n2722), ced-3(n1165); egl-1(n487), sem-4(n1378); ced-5(n2098) ced-3(n2721), sem-4(n1378); ced-5(n2098) ced-3(n2719), sem-4(n1378); ced-5(n2098) ced-3(n2830), ced-3(n2871) dpy-4(e1166), and ced-3(n3002) nIs50. For scoring mutant/+ animals, all the strains described above were crossed to wild-type animals and resulting heterozygous males were scored. The altered sequences in the ced-3 alleles n717, n718, n1040, n1129, n1163, n1164, n1165, n1286, n1949, n2426, n2430, and n2433 have been described previously (Yuan et al. 1993).

  • a Less than 15% of heterozygous animals contained one extra cell, and none contained more than one extra cell in the anterior pharynx.

  • b More than 50% of heterozygous animals contained at least one extra cell in the anterior pharynx.