TABLE 2

Residues required for CED-3 protease activity and CED-3 precursor processing are essential for ced-3 killing activity

ConstructLine no.No. ALMs/no. sides observed
Pmec-7lacZa140/40
Pmec-7ced-3(+)19/15
24/13
35/12
Pmc7ced-3(C358A)118/18
224/24
317/17
Pmec-7ced-3(C358S)122/25
225/26
314/14
Pmec-7ced-3(D374A)122/23
218/20
315/18
Pmec-7ced-3(D371A)110/20
213/20
313/20
Pmec-7ced-3(D371A, D374A)125/26
223/23
325/25
Pmec-7ced-3(D131A)17/20
28/19
312/20
Pmec-7ced-3(D221E)121/22
220/20
320/20
No transgene40/40
  • The amino acid alteration encoded by each transgene is in parentheses. Three transgenic lines were established independently for each transgene. All transgenes were injected into ced-9(n2812); ced-3(n7l7) animals to facilitate scoring of ALM survival (Shaham and Horvitz 1996a). +, wild type.

  • a Data from Shaham and Horvitz (1996a).