TABLE 3

Transformation frequencies and levels of sexual isolation using chimeric PCR-amplified DNA

Frequency of transformation (×10−3)Sexual isolation
Donor (source of chimera's insert)Wild-type recipient (1A96)ΔMutSL recipient (PB 1856)Wild-type recipient (1A96)ΔMutSL recipient (PB 1856)
B. subtilis subsp. subtilis 1A961.869 ± 0.214a3.663 ± 0.757a11
B. mojavensis ROC21.404 ± 0.1893.249 ± 0.7961.343 ± 0.023a1.210 ± 0.088a
B. atrophaeus NRRL NRS-2130.834 ± 0.0693.010 ± 0.7232.223 ± 0.0761.256 ± 0.054
B. licheniformis ATCC 145800.514 ± 0.0311.551 ± 0.3533.604 ± 0.2042.411 ± 0.067
  • a Mean and standard error values for four experimental trials.