TABLE 1

Primers for new TC1 polymorphisms

Marker/primerPositionaPrimer sequence (5′ to 3′)
pkP5062(I) + 11.35CAA TGA CCC TTT ACA TGT TCA G
pkP5036(I) + 26.76ACA TTT GCG TCG GGA ATC AC
zP598-L(III) − 2.5GTA GGC TTT GTA ACA AAG TAG TG
pkP580-U(X) + 15.10CGT AGT CAA CTT CTG CAG
Tcl.LlCAA GTC AAA TGG ATG CTT GAG
Tc1.L3CAT TTC GCT TTA TGC ACA CGG
  • a Estimated genetic map positions of Tc1 insertion polymorphisms based on physical map positions (ACEDB, Durbin and Mieg 1991), except for zP589-L, which is based on recombinations with flanking markers in our RI strains, as calculated by Map Manager (Manly and Cudmore 1994). Note that recombination distances observed in these RI strains are substantially different from those based on normal crosses (Shook et al. 1996).