TABLE 1

Strains and Plasmids

GenotypeReference or source
Strain
MG159BMATα ura3-52Brewster et al. (1993)
hog4-3MATa pbs2-3 (congenic with MG159B)Brewster et al. (1993)
W303-1AMATa ade2-1 his3-11 leu2-3,112 trp1-1 ura3-1 can1-100Laboratory stock
KDY1pbs2-3This work
KDY2pbs2-3 kss1Δ::URA3This work
KDY3pbs2-3 fus3Δ::LEU2This work
KDY4pbs2-3 slt2Δ::TRP1This work
KDY5pbs2-3 ste12Δ::URA3This work
KDY6pbs2-3 ste7Δ::URA3This work
KDY7pbs2-3 ste11Δ::URA3This work
KDY8pbs2-3 sho1Δ::URA3This work
KDY9pbs2Δ::LEU2This work
KDY10hog1Δ::TRP1This work
KDY11pbs2Δ::LEU2 kss1Δ::URA3This work
KDY12pbs2Δ::LEU2 ste7Δ::URA3This work
KDY13hog1Δ::TRP1 kss1Δ::URA3This work
KDY14hog1Δ::TRP1ste7Δ::URA3This work
KDY15pbs2-3 kss1Δ::URA3 fus3Δ::LEU2This work
KDY16pbs2-3 kss1Δ::URA3 slt2Δ::TRPThis work
KDY17pbs2-3 fus3Δ::LEU2 slt2Δ::TRPThis work
KDY18pbs2-3 kss1Δ::URA3 fus3Δ::LEU2 slt2Δ::TRPThis work
Plasmid
pRS426URA3 2μChristianson et al. (1992)
pRS423HIS3 2μChristianson et al. (1992)
pKD1URA3 2μ MSG5This work
pKD2URA3 2μ TRX2This work
pKD3URA3 2μ CAK1This work
pKD4HIS3 2μ MSG5This work
pKD5HIS3 2μ TRX1This work
pKD6HIS3 2μ TRX2This work
pKD7HIS3 2μ CAK1This work
pKD8pGEM-7ZF+ KSS1This work
pKD9pGEM-7ZF+ kss1Δ::URA3This work
pSL 1077pBR322 Ste7Δ::URA3Fields et al. (1988)
pSL 1094pBluescript KS+ ste11Δ::URA3Fields et al. (1988)
pSL 1311pSPGS ste12Δ::URA3Fields et al. (1988)
pYEE98pUC119 fus3-6Δ::LEU2Elion et al. (1990)
YEpT-KSS1TRP1 2μ KSS1Bardwell et al. (1996)
YEpT-kss1TRP1 2μ kss1 Y-F, T-ABardwell et al. (1996)
YEpL-FTyZLEU2 2μ Ty1::lacZCook et al. (1997)
YEpU-FTyZURA3 2μ Ty1::lacZCook et al. (1997)
  • All strains used in this study are congenic with W303-1A, with the exception of MG159B and hog4-3.