lir-1(RNAi) induces a phenotype only if lir-1 is in cis to lin-26
| Strain | dsRNA injected | Embryonic arrest (%) | Na |
|---|---|---|---|
| lir-1(mc33)mcD(3/lin-26(mc15)b | Uninjected | 48c | 193 |
| lir-1(mc33)mcD(3/lin-26(mc15)b | lir-1(exons1-4) | 50c | 133 |
| lir-1(mc33)mcD(3/lin-26(mc15)b | lin-26(full length) | 97 | 62 |
| rol-6(e187)d | lir-1(exons1-4) | 99 | 74 |
↵a Total number of progeny counted between 4 and 12 hr postinjection or cloning.
↵b Full genotype of the strain is lir-1(mc33) mcDf3 unc-4(e120)/lin-26(mc15) unc-4(e120).
↵c Uninjected animals laid 156 ± 55 eggs (N = 16); the progeny of injected animals laid 166 ± 46 eggs (N = 6); and in both cases egg counts were based on Egl+ animals (50% of the animals were Egl+).
↵d The injected control strain is CB187 (see materials and methods).