TABLE 2

lir-1(RNAi) induces a phenotype only if lir-1 is in cis to lin-26

StraindsRNA injectedEmbryonic arrest (%)Na
lir-1(mc33)mcD(3/lin-26(mc15)bUninjected48c193
lir-1(mc33)mcD(3/lin-26(mc15)blir-1(exons1-4)50c133
lir-1(mc33)mcD(3/lin-26(mc15)blin-26(full length)9762
rol-6(e187)dlir-1(exons1-4)9974
  • a Total number of progeny counted between 4 and 12 hr postinjection or cloning.

  • b Full genotype of the strain is lir-1(mc33) mcDf3 unc-4(e120)/lin-26(mc15) unc-4(e120).

  • c Uninjected animals laid 156 ± 55 eggs (N = 16); the progeny of injected animals laid 166 ± 46 eggs (N = 6); and in both cases egg counts were based on Egl+ animals (50% of the animals were Egl+).

  • d The injected control strain is CB187 (see materials and methods).