TABLE 2

Interaction between Rim11p and mutant Ime1p derivatives

GAD fusionaTwo-hybrid interaction with GBD-Rim11pb (β-galactosidase activity)c
None0.12
Ime1p2300
Ime1-L321Fp175
Ime1-R347Kp193
Ime1-Q340*p904
Ime1-E294*p0.1
Ime1-L321F, S360Fp833
Ime1-R347K, S360Fp815
  • a GAD-Ime1p has residues 1 to 360 of Ime1p.

  • b GBD-Rim11p has residues 1 to 370 of Rim11p.

  • c Strain Y190 was used in these assays. β-Galactosidase activity was determined in log phase cultures in SC-TRP-Leu medium. The values, in Miller units, are the mean of three determinations; all standard deviations were <25%.