TABLE 1

Yeast strains

StrainaGenotypeb
KB267arim11::LEU2/rim11::LEU2 ime2Δ4-lacZ::LEU2/+ met4/+ +/his4
M1085aRIM11-HA/RIM11-HA ime1Δ12::TRP1/ime1Δ12::TRP1 met4/+ +/his3
M1121aRIM11-HA/RIM11-HA ime1Δ12::TRP1/ime1Δ12:TRP1 ime2Δ4-lacZ::LEU2/+ met4/+ +/his4
YX282aime1Δ20/ime1Δ20 ume6Δ::TRP1/ume6Δ::TRP1
YX306aume6Δ::TRP1/ume6Δ::TRP1
YX471arim11:LEU2/rim11::LEU2 ume6Δ::TRP1/ume6Δ::TRP1
Y187α gal4 gal80 his3 trp1-901 ade2-101 leu2-3, 112 ura3-52::URA3-GAL1-lacZ
Y190a gal4 gal80 his3 trp1 ade2 ura3 leu2 URA3::GAL1-lacZLYS2::GAL1-HIS3 cyhr
  • a All strains except Y187 and Y190 are derived from strain SK-1.

  • b All haploid SK-1 derivatives have additional markers ura3 leu2 try1 lys2 ho::LYS2 gal80::LEU2, and diploid SK-1 derivatives are homozygous for those markers.