TABLE 8

Distribution of donor and “restrictionless-recipient” DNA in transconjugants

A. Conjugation: ECOR 47 → ER 2476
Minutes
75//22242527.5--28-----------------------------------------------------------------29------------30--
+HHPNAGGBOLASCEBSTAATN
YTOADOLUNKLQ*BDSBACHYR
TransARTR#
15+----DDDDDDDD*DDDDDDDDD(1)
  2, 24+-----DDDDDDD*DDDDDDDDD
12, 23+-----------D*DDDDDDDDD
13+-------DDDDD*DDDDDDDD-
16+-----------D*DDDDDDDD-
27+-----DDDDDDD*DDDDDDD--
30+-----------D*DDDDDDD--
  3+----DDDDDDDD*DDDDDD---
  1, 22+-----------D*DDDDDD---
20+---------/dDDD*DDDDD----
21+------------*Dd/-DDDD---
25+------------*DDDDD----
  5, 10+------DDDDDD*DD-------
17+-----DDDDDDD*D--------
  7+--------DDDD*D--------
18+----------DD*Dd/--------
29+-----------D*Dd/-------
19+----------DD*D--------
  4, 6, 11+-----------D*---------
  9+------------*---------
14+D-----------*D--------(2)
26+-DD---------*DD-------
  8+--D------/dDDD*DDDDDD---
28+----DDDDd/---D*---------
B. Back Conjugation: (ECOR 47 → ER2476) → ER2476
Minutes
90//2527.5---28---------------------------------------------------------------29-------------30--
+PNAGGBOLASCEBSTAATN
OADOLUNKLQ*BDSBACHYR
TR
[Donor HLZ-15--DDDDDDDD*DDDDDDDDD]
Trans#
3, 5-8, 11-15+-DDDDDDDD*DDDDDDDDD(1)
4+-DDDDDDDD*DDDDDDD---
1, 9, 10+-----DDDD*DDDDDDDDD
2+-DDDDDDDD*DD-------(2)
  • *, selected marker, trpA+; + , counterselected marker, rpsL (at 75 min, top) or rpoB (at 90 min, bottom). Other symbols as in Table 2.