TABLE 6

BLASTP matches from the CI and CII trp regions

GeneProtein name/predicted functionMatching speciesCodonsa% Identicalb% PositivescE valued
CI trpE-yibQ-trpDCG region
asmAeProtein assemblyE. coli15023478-04
ybaUProtease maturation proteinE. coli62124421e-30
trpEfAnthranilate synthase (Component I)R. sphaeroides50399990.0
yibQHypothetical HI0755H. influenzae26622390.094
trpGAnthranilate synthase (Component II)Azospirillum brasilense19571792e-71
trpDAnthranilate phosphoribosyltransferaseBradyrhizobium japonicum33857722e-106
trpCIndoleglycerolphospate synthaseA. brasilense27064751e-87
moaCMolybdopterin biosynthesisBr. japonicum15962758e-50
moeAMolybdopterin biosynthesisE. coli39434508e-54
lexA/dinRSOS response regulatorB. subtilis22834569e-30
comECompetence proteinB. subtilis72926447e-27
gltXGlutamyl-tRNA synthetaseRickettsia prowazekii4714364e-116
cisYeCitrate synthaseBr. japonicum4256733e-05
CII trpF-aroR-trpB region
cmkACytidylate kinase 1H. influenzae20641605e-38
rpsA1Ribosomal protein S1Rhizobium meliloti56165770.0
hipIntegration host factor β-subunitR. capsulatus9383907e-38
trpFPhosphoribosylanthranilate isomeraseCaulobacter crescentus21246611e-45
aroRRegulatorStreptomyces griseus48648e-31
trpBTryptophan synthase β chainPseudomonas aeruginosa4097383e-173
orfUnknownSynechocystis sp.12457697e-33
CI trpA region
gtpPGTP binding proteinB. subtilis3695572e-115
trpATryptophan synthase α chainP. putida26358738e-86
guaBeInosine-5′-monophosphate dehydrogenaseMethanobacterium thermoautotrophicum7138631e-06
lctDLactate dehydrogenaseNeisseria meningitidis3876582e-143
mosCTransporterRhizobium sp. NGR23438525426e-28
  • a The number of codons (amino acid residues) that encode the R. sphaeroides gene (excluding the stop codon).

  • b,c Percentage of amino acid identity and similarity between the predicted translation of the R. sphaeroides protein and the protein in the database that showed the best BLASTP match.

  • d Expect value given from an unfiltered BLASTP search.

  • e This gene has not been fully sequenced.

  • f The second best match was to P. putida TrpE (493 codons). Identity, 51%; Positive, 64%; Expect, e-125.