TABLE 1

Molecular markers across chromosome 2 of D. pseudoobscura

LocusGenBank
accession no.
PrimersTemp.Type of variantLocationNo. of alleles
in 27 lines
Cytological
locationa
Dps2003Not availableLengthUnknownNot determined43
trop1AF039274F:202-22358°Length: (CT)nIntron443/44 border
R:388-409
Rh3X65879F:1673-169458°Length3′ UTR644
R:1890-1869
GldM29299F:2101-212165°Length:(CTGA)nIntron>645
R:2240-2221
Rh1X65877F:2131-215065°Length:(CAA)n3′ UTR>745
R:2386-2365
bcdX55735F:1402-142266°Length:(CAG)nCoding350
R:1622-1603
UbxX05727F:1488-150959°SSCPIntron4ND
R:1665-1643
XdhM33977F:1883-190259°SSCPIntron>1054/55 border
R:2105-2086
Mlc1L08052F:991-101158°Length: (CA)nIntron659/60 border
R:1130-1111
rp49S59382F:808-82860°SSCP5′ UTR262
R:1163-1144
  • ND, not determined.

  • a Location of Dps2003 is by inference from genetic data; trop1, Gld, and Mlc1 were determined by B. Charlesworth (personal communication); bcd, Xdh, and rp49 are from Segarra et al. (1996); Rh1 is from Carulli and Hartl (1992); Rh3 is from this study (see text).