TABLE 1

Yeast strains

StrainGenotype (or parents)
AS4MATα trp1 arg4 tyr7 ade6 ura3a
AS13MATa leu2 ade6 ura3a
DNY47AS13 his4-IR9 (18-bp palindromic insertion at +467)b
PD100AS13 his4-IR9 his4-3133 (18-bp palindromic insertion at +467; 26-bp palindromic insertion at +2327)c
PD98AS13 his4-3133 (26-bp palindromic insertion at +2327)d
KY26AS4 his-713 [1-bp insertion (G) at +2270]
KY29AS4 his4-3133 (26-bp palindromic insertion at +2327)
KY44KY26 SpeI:URA3 [URA3 gene inserted downstream (CEN-distal) from His4]
KY45AS4 SpeI:URA3
KY46DNY47 SpeI:URA3
KY47PD100 Spel:URA3
KY48DNY47/KY44
KY49KY46/KY26
KY51KY47/AS4
KY52KY46/KY29
KY53KY46/AS4
KY54AS13/KY44
KY55PD98/KY45
Tester strainse
PD21AS13 his4-Δ29 (his4a his4b HIS4C)c
PD68AS4 his4-Δ29 (his4a his4b HIS4C)c
KY32MATa ade2 his4-713 (HIS4A HIS4B his4c)
KY33MATα ade2 his4-713 (HIS4A HIS4B his4c)
KY42MATa ade2 his4-3133 (HIS4A HIS4B his4c)
KY43MATα ade2 his4-3133 (HIS4A HIS4B his4c)
KY56MATa ade2 his4-Δ29 (his4a his4b HIS4C)
KY57MATα ade2 his4-Δ29 (his4a his4b HIS4C)
  • a Stapleton and Petes (1991).

  • b Nag and Petes (1991).

  • c Detloff and Petes (1992).

  • d Detloff et al. (1992).

  • e Tester strains were used for allelism tests as described. PD21 and PD68 were obtained from Tom Petes’ laboratory (University of North Carolina). All other tester strains are segregants from crosses between various lab strains, selected for the presence of desired alleles. Other alleles are uncharacterized.