TABLE 5

Quantitative complementation tests

Sternopleural bristlesAbdominal bristles
MutationH(M - C)aL(M - C)bC × LcH(M - C)L(M - C)C × L
sc-0.68-0.65NS-2.93-1.78*
spl-0.68-1.00NS-0.300.90*
ct6-0.35-0.30NS-0.68-0.33NS
emcP(3)50.74-0.28**-0.13-0.79NS
emcP(3)670.880.30NS0.600.24NS
qm1.30-0.09***0.020.22NS
h1.21-0.05***1.080.41NS
abd0.06-0.83**-0.85- 0.98NS
pyd0.79-0.75***0.570.99NS
Scr2.701.83*-1.73-1.44NS
D11.63-0.76***1.652.62NS
H-3.76-3.20NS-3.17-3.06NS
E(spl)0.21-0.38*-0.51-0.25NS
  • NS, P > 0.05; *, 0.10 > P > 0.05; **, 0.05 > P > 0.01; ***, 0.01 > P > 0.001; ****, P < 0.001.

  • a The difference in bristle number between heterozygotes for mutant and wild-type (control) alleles at the candidate loci in the high-selection line background, averaged over sexes and replicates.

  • b The difference in bristle number between heterozygotes for mutant and wild-type (control) alleles at the candidate loci in the low-selection line background, averaged over sexes and replicates.

  • c The results of tests of significance of the cross × line interaction term from four-way factorial analyses of variance of bristle number, in which variance in bristle number was partitioned into sources attributable to the main effects of cross, C (mutant or control allele at the candidate locus); line, L (high or low selection line); sex, S; and replicate, R; and all their interactions. The C × L term tests whether the complementation effect [H(M - C) - L(M - C)] is significantly different from zero.