TABLE 2

Screen summaries

ScreenReferenceaElementRaw IILocalized IISingle IIIn Df IIGroup IIPrimary IIGene II
Spradling1Phsneo2222111
Spradling2PZ[ry]495479432292291240233
Rubin3PZ[ry]504742341176
Jan4PlacW1111111
Scott5PlacW3433331915129
Kiss6PlacW215316111133712855351302
Laughton7PlacW
Totals27352173164310601174612552
ScreenPercentage in Df verifiedScreen efficiencyRaw IIILocalized IIISingle IIIin Df IIIGroup IIIPrimary IIIGene III
Spradling636168686635452321
Spradling8578609597560361331257248
Rubin787696949455432423
Jan8883201190185124846461
Scott635079675934372018
Kiss5831
Laughton48391251168848744532
Totals117811321052657614433401

The results of analyzing lines from seven single P-element autosomal insertion screens are summarized, grouped by chromosome (II and III). raw, number of starting lines; Localized, number of lines whose P insertions were localized by in situ hybridization; single, number of lines with a single P insertion; in Df, number of lines whose insertion fell within the cytogenetic boundaries of an available valid deficiency; Group, number of different complementation groups; Primary, number of lines in the primary collection. Note: The distribution of lines to the primary collection from the different screens is essentially arbitrary and was determined by the relative time each screen was analyzed as indicated by the order in which the screens are listed in the table. Genes, estimated number of different genes disrupted; % in Df verified, fraction of lines in valid deficiencies that were verified; Screen efficiency, the fraction of raw lines in a screen that are verifiable single insertions. Efficiency was calculated using percentage verified in Df to estimate the fraction of single insert lines outside of Dfs that would have been verified. The formula is eff = [verified + (% in Df verified) [not in Df and single insert]]/raw.

  • a 1, Cooley et al. (1988); 2, Karpen and Spradling (1992); 3, Gaul et al. (1992); 4, Bier et al. (1989); 5, M. Scott and M. Fuller, personal communication; 6, Törok et al. (1993); 7, Chang et al. (1993).