TABLE 1

Effects of Cb-her-1 and Bm-her-1 ectopic expression in C. briggsae and C. elegans

Transgenic roller
progeny produced
Injected strainInjected transgeneTotalMaleaIntersexb
C. elegans N2Cb-her-157570c
C. briggsae AF16Cb-her-143d376
C. briggsae AF16Cb-her-1FSe3900
C. elegans N2Bm-her-151304
C. elegans PA43Bm-her-14800
C. briggsae AF16Bm-her-17910
  • a Male refers to animals that appeared under the dissecting microscopeto havea male-like tail andnoobvious hermaphroditic characteristics.

  • b Intersex refers to animals with both a male-like tail and a hermaphrodite vulva, based on scoring with a dissecting microscope.

  • c We observed two non-Roller intersex progeny, which must have been transgenic animals because this phenotype is never seen among progeny of uninjected animals.

  • d We observed an additional 328 non-Roller male-like progeny, of which 70 were intersexes. These must also have been transgenic animals because these phenotypes are never seen among progeny of uninjected animals.

  • e A derivative of the Cb-her-1 construct that contains a frameshift mutation in exon 2.