TABLE 1

Comparison of ARS activity in S. cerevisiae and S. monacensis

ARS elementMutationS. cerevisiae phenotypeaS. monacensis phenotypea
ARS307carl NoneArs+Ars+
ACSArsArs
ARS305carl NoneArs+Ars+
ACSArsArs
Box 3′ArsArs
B1ArsArs
ARS310carl NoneArs+Ars+
ACSArsArs

Mutations refer to the mutations described in Figures 3 and 4. pRS333 plasmids carrying the ARS construct indicated were used to transform S. cerevisiae and S. monacensis to G418 resistance.

  • a Ars+ indicates that transformants were obtained at high frequency, while Ars indicates that transformants were obtained rarely or not at all.