TABLE 3

Effect of dun1, rad52, and rad1 mutations on intrachromosomal recombination

StrainGenotypeaRateb% Pop-outs (total)cRatiod
YA156 DUN1 5.3 × 10–457 (533 Ura/966 Ade+)1
YD100 dun1::HIS3 6.2 × 10–43.7 (32 Ura/867 Ade+)0.06
YD102 rad52 ::TRP1 4.0 × 10–571 (898 Ura/1271 Ade+)1.25
YD110 rad52::TRP1 dun1::HIS3 5.4 × 10–522 (274 Ura/1267 Ade+)0.4
YD108 rad1::LEU2 9.1 × 10–53.9 (99 Ura/2594 Ade+)0.07
YD111 rad1::LEU2 dun1::HIS3 2.5 × 10–45.5 (66 Ura/1189 Ade+)0.1
  • a For full genotype, see Table 1.

  • b Rate, number of events/cell division.

  • c Data from four independent experiments.

  • d Percentage pop-outs in mutant/percentage pop-outs in wild type.