TABLE 1

Plasmids used in this study

Description/applicationReference
Vectors
    pET11aApr, T7 promoter and terminatorDubendorff and Studier (1991)
    pBluescript KS/SKApr, lac′ IPOZ′ α, T7 promoterShort et al. (1988)
    pUC18/19Apr, lac′ IPOZ′ αYanisch-Perron et al. (1985)
Recombinant plasmids
    pET11a constructs
        pMjaL1.4Overproduction of MjaL1Köhrer et al. (1998)
        pMthL1.4Overproduction of MthL1Köhrer et al. (1998)
        pMvaL1.55M5Overproduction of MvaL1Mayer et al. (1998)
        pSacL1.4Insert: 0.7-kb NdeI-BamHI PCR fragment (template:
genomic DNA of S. acidocaldarius), containing the
SacL1 gene; overproduction of SacL1
This study
        pSsoL1.4Overproduction of SsoL1Köhrer et al. (1998)
    pBluescript expression constructs
        pMjaL1.3Insert: 0.8-kb SspI-AflIII fragment containing the
MjaL1 gene; in vitrosynthesis of MjaL1 (regulation
studies)
This study
        pMjaL1.3M5Derivative of pMjaL1.3 with M5 mutationThis study
        pMvaL1.35In vitro synthesis of MvaL1 (regulation studies)Hanner et al. (1994)
        pMvaL1.35M5Derivative of pMvaL1.35 with M5 mutationMayer et al. (1998)
    pBluescript transcription constructs
        pMthL1.23SInsert: 0.15-kb SacI-HindIII PCR fragment (tem-
plate: genomic DNA of M. thermolithotrophicus)
containing the MthL1 binding site on 23S rRNA;
in vitro synthesis of a 150-nt-long 23S rRNA
fragment
This study
        pMvL1.23SIn vitro synthesis of a 210-nt-long M. vannielii 23S
rRNA fragment containing the MvaL1 binding site
Mayer et al. (1998)
        pSacL1.23SInsert: 0.16-kb SacI-HindIII PCR fragment
(template: genomic DNA of S. acidocaldarius)
containing the SacL1 binding site on 23S rRNA;
in vitro synthesis of a 160-nt-long 23S rRNA frag-
ment
This study
        pMthL1.TkInsert: 0.25-kb SacI-HindIII PCR fragment (tem-
plate: genomic DNA of M. thermolithotrophicus)
containing the MthL1 binding site on mRNA; in
vitro synthesis of a 250-nt-long mRNA fragment
This study
        pMthM5.TkDerivative of pMthL1.Tk with M5 mutationThis study
        pMvL1L10In vitro synthesis of a 250-nt-long M. vannielii mRNA
fragment containing the MvaL1 binding site
Mayer et al. (1998)
        pMvM5L10Derivative of pMvL1L10 with M5 mutationMayer et al. (1998)
        pSacL11.TkInsert: 0.25-kb SacI-HindIII PCR fragment
(template: genomic DNA of S. acidocaldarius)
containing the potential SacL1 binding site on
L11 mRNA; in vitro synthesis of a 250-nt-long
mRNA fragment
This study
        pMva16S-S8BS.3In vitro synthesis of a 220-nt-long M. vannielii 16S
rRNA fragment containing the MvaS8 binding site
Köhrer et al. (1998)