TABLE 1

Levels of glycosyl hydrolases in the S. solfataricus mutant isolates

Glycosyl hydrolase activities
Strainaβ-Glycosidase
(μmol PNPb/min/mg)
α-Glucosidase
(μmol PNP/min/mg)
α-Amylase
(units/ml)
Wild type31.7 ± 0.312.7 ± 0.91.15 ± 0.03
Mutant 1 (Car1)1.0 ± 0.1 (32)c12.3 ± 1.7 (1)0.19 ± 0.02 (6)
Mutant 2 (Car2)1.1 ± 0.6 (29)25.6 ± 1.8 (2)0.17 ± 0.01 (7)
Mutant 4 (lacS100)<0.00114.3 ± 3.7 (1)1.13 ± 0.02 (1)
Mutant 5 (Car5)0.9 ± 0.1 (35)10.0 ± 0.8 (1)0.18 ± 0.02 (6)
  • a Names in parentheses refer to phenotypic and genotypic designations.

  • b PNP is para-nitrophenol.

  • c Values in parentheses indicate the fold change in specific activity relative to wild type rounded to the nearest integer.