TABLE 4

Interaction of Abp1 PRO and Las17p with Rvs167p in the two-hybrid system

PlasmidpAS1 Miller unitsapAS1-ABP1 PropAS1-LAS17/BEE1pAS1-PCL2
Miller unitsFold increaseMiller unitsFold increaseMiller unitsFold increase
pAD0.05 ± 0.020.01 ± 0.020.2b000.06 ± 0.091.2
pAD-RVS1670.10 ± 0.11365 ± 240c7306346 ± 159c6915NDd
pAD-P473L0.10 ± 0.07000.32 ± 0.496.491.9 ± 17.01838
pAD-BARGPA0.02 ± 0.030000ND
pAD-GPASH30.07 ± 0.133.39 ± 0.4967.8270 ± 1185406ND
pAD-BAR00.01 ± 0.020.200ND
pAD-SH30.30 ± 0.073.43 ± 1.4368.6179 ± 16335840.23 ± 0.094.6
  • a β-Galactosidase activity—see materials and methods and Andrews and Moore (1992); average of three independent cultures.

  • b Fold increase is calculated by dividing the Miller units of the pAS1-fusion pAD-fusion interaction value by the interaction value of pAS1 pAD interaction (0.05).

  • c Average of best three out of four values.

  • d Not done.