TABLE 3

Interaction of Rvs167p and Rvs161p with Rvs167p in the two-hybrid system

PlasmidpAS1 Miller unitsapAS1-RVS161pAS1-RVS167ΔSH3pAS1-BAR
Miller unitsFold increaseMiller unitsFold increaseMiller unitsFold increase
pAD0.05 ± 0.0200b0.09 ± 0.041.80.11 ± 0.042.2
pAD-RVS167026.0 ± 10.552112.3 ± 4.79c2472.40 ± 0.5948.0
pAD-BARGPA05.43 ± 0.69d1095.08 ± 0.421022.35 ± 0.8147.0
pAD-GPASH30.03 ± 0.04000.08 ± 0.11c1.60.09 ± 0.031.8
pAD-BAR0.10 ± 0.068.57 ± 3.611713.36 ± 0.9667.21.31 ± 0.1226.2
pAD-PCL2000NDeND
  • a β-Galactosidase activity—see materials and methods and Andrews and Moore (1992); average of three independent cultures.

  • b Fold increase is calculated by dividing the Miller units of the pAS1-fusion pAD-fusion interaction value by the interaction value of pAS1-pAD (0.05).

  • c Average of best three out of five values.

  • d Average of best three out of six values.

  • e Not done.