TABLE 2

Interaction of Pcl2p with Rvs167p in the two-hybrid system

PlasmidpAS1 Miller unitsapAS1-PCL2 Miller unitsFold increase in activation
pAD0.07 ± 0.030.47 ± 0.076.7b
pAD-RVS1670.20 ± 0.1674.5 ± 5.40372
pAD-BARGPA0.04 ± 0.0328.0 ± 5.61700
pAD-GPASH30.06 ± 0.053.39 ± 0.0956.5
pAD-BAR0.14 ± 0.020.64 ± 0.104.6
  • a β-Galactosidase activity—see materials and methods and Andrews and Moore (1992); average of three independent cultures.

  • b Fold increase is calculated by dividing the value of the PAS1-PCL2 pAD-fusion interaction by the pAS1 pAD-infusion interaction value.