Segregation for kanamycin resistance in the selfed progeny (F1) of the tobacco lines, with a kanamycin gene restored by homologous recombination.

LineKanrkansr/s χ 2
1-12 ASensitive
1-12 A 1163572.9/10.05
1-12 A 2107402.7/10.16
1-12 BSensitive
1-12 B 1185722.6/10.64
1-12 B 2128482.7/10.24

The χ2 values indicate that the ratios do not deviate significantly from Mendelian segregation (rejection P < 0.05).

Kanr, resistant; kans, sensitive; r/s, ratio of kanamycin resistant to sensitive seedlings.