TABLE 1

DSB-induced ectopic homologous recombination

Transgene lineI-SceIHR KanraTransformed cells (T)b × 105 (seedlings)Frequency H/T
1-12A (5 experiments)02.0 (2007)< 5.0 × 10–6
+21.9 (1905)10.5 × 10–6
1-12B (11 experiments)04.0 (3984)< 2.5 × 10–6
+23.9 (3940)5.1 × 10–6

HR, homologous recombinants; T, transformants.

  • a Kanamycin-resistant calli in which the kanamycin gene was restored by homologous recombination as shown by Southern hybridization. The calli were obtained from independent experiments.

  • b Based on the estimation of 100 transformation events per seedling as demonstrated in previous experiments (Tinland et al. 1995; Puchta 1998).