TABLE 1

Description of the 26 independent de novo mutations observed in a microsatellite survey of Syngnathus typhle

Male IDCluster sizebNo. progeny assayedGenotypeMutationaGermline origin
FatherMotherEmbryo
Single mutations at typh04
MT21-2170197/213175/233213/229233→229Maternal
MT22-1125205/205167/205167/209205→209Paternal
MT22-213217/229209/233217/229233→229Maternal
MT26-1c110205/237201/237237/241237→241Undetermined
MB2-2131185/213213/269189/269185→189Paternal
MB-8214189/237205/221209/237205→209Maternal
MT1-2123213/229171/217209/217213→209Paternal
MT1-3147175/241193/201175/197193d→197Maternal
FCST2123191/209197/null197/217209→217Paternal
FCST6160213/221205/217217/217213→217ePaternal
FCST8130171/201225/237201/233237→233Maternal
FCST11142197/245201/205201/237245→237Paternal
FCST12161205/225189/217189/229225→229Paternal
FCST20142189/229171/229185/229189→185Paternal
FCST27115193/213179/221213/225221→225Maternal
FCST3415183/185193/225185/213225→213Maternal
Clustered mutations at typh04
MT11-1513189/213197/209197/217213→217Paternal
209/217213→217Paternal
185/229185/217213→217Paternal
185/217213→217Paternal
217/229213→217Paternal
MT19-1233209/233213/217213/229233→229Paternal
217/229233→229Paternal
FCST20342189/229171/229171/225229→225Paternal
171/225229→225Paternal
171/225229→225Paternal
FCST26259185/217191/205189/205185→189Paternal
189/205185→189Paternal
Single Mutations at typh16
MT3-2123235/267247/275271/275267→271Paternal
MB6-4118263/267235/255259/267255→259Maternal
MT29-1115259/271235/251239/259235→239Maternal
FCST217225/263251/271247/263251→247Maternal
FCST55150247/307259/263263/303307→303Paternal
Clustered mutations at typh16
MT29-129259/271235/235231/271235→231Maternal
231/271235→231Maternal
  • a Lengths of the ancestral and derived (mutant) sequences, in base pairs.

  • b Observed numbers of offspring carrying a copy of the same mutant allele.

  • c Which parent produced the mutant gamete could not be determined in this case, because both the father and mother carried the 237 allele.

  • d Alternatively, the original allelic state could have been 201, but in either case only one 4-bp change was involved.

  • e Alternatively, the original allelic state could have been 221, but in either case only one 4-bp change was involved.