TABLE 6

Effect of parental developmental temperature on the imprinting of the garnet+ gene of the Dp(1;f)LJ9 mini-X chromosome

Parent culture temperatureProgeny culture temperature
18°22°25°29°
18°MAT94 ± 389± 363± 533± 10
PAT42 ± 437± 250± 339± 2
22°MAT85 ± 487± 361± 249± 2
PAT27 ± 336 ± 354± 330± 3
25°MAT80 ± 496± 458± 354± 1
PAT30 ± 234± 427± 241± 2
29°MAT91 ± 592± 3085 ± 459± 4
PATNDNDNDND

The effect of developmental temperature on the pigmentation of y za g53d/Dp(1;f)LJ9 males. The origin of the mini-X chromosome is shown on the left (MAT, maternally derived mini-X chromosome; PAT, paternally derived mini-X chromosome). The rows give the effect of temperature on the progeny (effect on variegation) and the columns show the effect of temperature on the parents (effect on imprint). Expression of the garnet gene was monitored as described in materials and methods. Data are given as percentage wild-type garnet eye pigment expression ±SEM, as described in materials and methods. ND, not done.