TABLE 2

Summary of results from Southern blotting analyses of recombinants

DNA substrateNo. of recombinants analyzedNo. of gene conversionsaNo. of mutantsbNo. of duplicationscFraction coconvertedd
pAA-TC1914505/10
pAG-TT423660ND
pAG-TG87010/8
pAG-AG76100/6
pAG-AT5113ND

ND, not done.

  • a Number of HATR segregants that had the expected pattern for a simple gene conversion.

  • b Number of HATR segregants that did not lose the XhoI linker insertion and presumably acquired a second compensating mutation.

  • c Number of HATR segregants that, in addition to having undergone a gene conversion, displayed an increase in copy number of the recombination substrate possibly resulting from nondisjunction or amplification.

  • d First number (before the slash) is the number of clones that showed coconversion of the SacI site along with correction of the XhoI linker insertion mutation. The second number is the number of clones tested.