TABLE 3

Target sites for illegitimate recombination in S. cerevisiae and C. glabrata

C. glabrataS. cerevisiae
No. of eventsTop1No. of eventsTop1
NED9(0.53)16(0.26)5
SED8(0.47)47(0.30)4
BED010(0.44)2
Total1752311
  • Classification of homology between the ends of the integrating plasmid and the target site in the genome. The events are classified according to the system of Schiestl et al. (1994). The SED (single end directed), NED (neither end directed), or BED (both ends directed) classification is based on how many ends of the integrating plasmid have homology with the target site. For the sake of consistency and comparison with the work of Schiestl et al. (1994) in S. cerevisiae, we count as homologous those events involving at least two base pairs immediately adjacent to the recombination point; a single nucleotide of homology (for example, insertion 1) is not counted as microhomology because this will occur on a purely random basis. The Top1 column summarizes the frequency of finding the Top1p consensus recognition site [(C/G)(A/ T)T] immediately adjacent to the insertion point. The data for S. cerevisiae, shown for purposes of comparison taken from Figure 1A (Schiestl et al. 1994), Figure 5 (Schiestl and Petes 1991), Figure 1 (Schiestl et al. 1993), and Figure 4 (Schiestl et al. 1993).