The bob1-1(mcm5) mutation does not suppress a null mutation in rad53Δ::URA3
| Relevant genotype of strain dissected | Type of tetrad | Number |
|---|---|---|
| A. bob1-1 or second site suppressor suppresses rad53Δ::URA3 mutation | ||
| bob1/ + + /rad53Δ::URA3 | 4:0 viable:inviable | 0 |
| (299 × P119) | 3:1 viable:inviable | 9a |
| 2:2 viable:inviable | 16 | |
| Total tetrads dissected | 25 | |
| B. Backcross to determine if viable segregants are suppressed by bob1-1 or by a second site suppressor | ||
| bob1/bob1 +/rad53Δ::URA3 sup?/+ | 4:0 viable:inviable | 13 |
| 3:1 viable:inviable | 7 | |
| 2:2 viable:inviable | 4 | |
| Total tetrads dissected | 24 | |
a Viable bob1-1 rad53Δ::URA3 from part A was backcrossed to bob1-1 strain.