TABLE 2

List of plasmids used in this study

PlasmidsDescriptionSource
pBluescriptKS +Cloning vectorStratagene
pRS314TRP1 yeast shuttle vectorSikorski and Hieter (1989)
pRS315LEU2 yeast shuttle vectorSikorski and Hieter (1989)
pRS316URA3 yeast shuttle vectorSikorski and Hieter (1989)
pPD4pRS316-CDC7-ADE3 (URA3)This study
pPD7pRS314-CDC7-ADE3 (TRP)This study
pPD32pRS315-DBF4 (LEU2)This study
pGO117pRS425-DBF4 (LEU2)This study
pRS277pCDC7 CEN (LEU2)Hollingsworth et al. (1992)
pRS288pcdc7-1 CEN (LEU2)Hollingsworth et al. (1992)
pPD58YCp50-RAD53 genomic cloneThis study
pPD60pRS316-RAD53 (URA3)This study
pPD83pRS314-RAD53 (TRP1)This study
pBR322-pep4Δ::URA3pep4Δ::URA3 disruption plasmidGift from B. Tye
pNKY51phisG::URA3::hisG cassetteAlani et al. (1987)
pPD84pBS-rad53Δ::hisG-URA3-hisGThis study
pY2pGAL4DB (TRP1)Sadowski et al. (1992)
pPD93pGAL4DB-RAD53i (aa50-aa457) fusion (TRP1)This study
pPD94pGAL4DB-RAD53 (aa50-aa822) fusion (TRP1)This study
pGO109pGAL4DB-DBF4 fusion (TRP1)Shellman et al. (1998)
pGAD-2FpGAL4AD (LEU2)Chien et al. (1991)
pGO119pGAL4AD-CDC7 fusion (LEU2)Shellman et al. (1998)
pCH441pGAL4AD-DBF4 fusion (LEU2)Hardy and Pautz (1996)
pGO174pRS316-7HA-DBF4 (URA3)This study
pGAP-RNR1pRNR1 driven off GAP promoter (TRP1)T. Weinert