TABLE 4

Polarization of swo mutants

MutantG.T.a at 42°?Downshiftb (42° → 30°)Upshiftc,d (30° → 42°)
swoA-1 NoMultiple hyphae, “axial”eBasalf swells, new apicalg growth swells
swoA-2 NoMultiple hyphae, “axial”Basal swells, no new apical growth
swoC NoNo hyphaeBasal swells slightly, new apical growth normal
swoD NoNo hyphaeBasal swells slightly, new apical growth normal
swoF NoNo hyphaeBasal swells slightly, new apical growth swells
swoB YesBasal swells slightly, apical normalBasal swells, apical swells slightly, abnormal branching
swoE YesBasal swells, apical normalBasal swells, apical swells at tip
swoG YesBasal swells, apical normal, hyperbranchingBasal normal, apical normal
swoH YesBasal swells, apical normalBasal normal, apical normal
  • a Germ tube; see Figure 2.

  • b Conidia were inoculated onto coverslips in liquid medium, incubated at restrictive temperature for 10 hr, shifted to permissive temperature, and incubated for another 2 hr; see Figure 4.

  • c Conidia were inoculated onto coverslips in liquid medium, incubated at permissive temperature for 10 hr, shifted to restrictive temperature, and incubated for another 2 hr; see Figure 5.

  • d For the swo mutants that do not make germ tubes at 42°, antibodies against total A. nidulans cell walls were used to distinguish growth that occurred before and after the temperature shift; see Figure 6.

  • e All germ tubes emerged from one side of the conidium. Whether these germ tubes arise sequentially and immediately adjacent to each other has not yet been determined.

  • f The older end of the germling, the conidium end.

  • g The newer end of the germling, the hyphal tip end.