TABLE 3

Nuclear number for nonpolar swo mutants

MutantAverage nuclear numberaHigh nuclear numberLow nuclear numberNuclear morphologyb
swoA-2 4010813Normal
swoC 5.8122Elongated, stringy
swoD 5.2102Normal
swoF 5.3122Elongated, stringy
  • a Conidia were inoculated onto coverslips in liquid medium, incubated at restrictive temperature for 13 hr, fixed, and stained with Hoescht's 33258 to visualize nuclei. Nuclei in 50 germlings were counted. Two independent experiments gave similar results. The average, high, and low nuclear numbers represent all 100 germlings counted. Because swoA and swoC mutants are thick, accurate nuclear counts are difficult (see Figure 2). Therefore, counts for these strains may underrepresent the total nuclear number.

  • b Abnormal nuclear morphologies in swoC and swoF made accurate nuclear counts difficult. Therefore, counts for these strains may underrepresent the total nuclear number.