TABLE 3

Transformation rescue of smg-7(r1131)

Motility phenotype following:smg-7 Activity following heat shock
Host genotypeInjected DNANo heat shockHeat shock
unc-54(r293) NoneParalyzedParalyzed+
unc-54(r293); smg-7(r1131) NoneMotileMotile
unc-54(r293); smg-7(r1131) hsp16-2::smg-7(+) MotileParalyzed+
wild type hsp16-2::smg-7(+) MotileMotile+

Heritable transformants were heat shocked at 33° for 50 min, after which individual larvae were isolated, grown at 25° for 1 to 2 days, and scored for motility.