TABLE 2

Rates of minisatellite instability in wild-type, pol3-t, pol30-52 and pol30-52 pol3-t double-mutant haploid strains after growth at 25°

GenotypeRate of instability/cell divisionaNormalized rate of instability
Wild type9.0 × 10–5 (7.7–12 × 10–5)1
pol3-t 2.2 × 10–4 (1.6–3.1 × 10–4)3
pol30-52 4.1 × 10–4 (3.5–6.4 × 10–4)5
pol30-52 pol3-t 2.1 × 10–3 (1.2–5.3 × 10–3)23

Unlike the data shown in Table 1, these data were obtained in experiments in which the strains were grown at 25°.

  • a Rates for the wild-type, pol3-t, and pol30-52 strains were based on an analysis of 20 independent cultures. Fluctuation analysis for the pol30-52 pol3-t strain was performed as described in materials and methods. Numbers in parentheses represent 95% confidence intervals.