TABLE 1

Rates of microsatellite and minisatellite instability in wild-type, msh2::Tn10LUK, and pol30-52 strains

Repeat size × repeat no.aRelevant genotypebRate of 5-FOAR cells in independ. expts. (10–5)cAverage rate of tract changes (10–5)dFold increase vs. wild type
1 × 18Wild type0.59 (0.36–1.3)0.671
0.80 (0.70–1.1)
0.61 (0.40–0.68)
msh2 3900 (1900–5500)41006100
4200 (1100–6000)
pol30-52 1500 (920–2300)12001800
910 (670–2000)
2 × 16.5Wild type0.49 (0.37–0.97)0.481
0.51 (0.36–0.76)
msh2 150 (120–170)160340
150 (110–270)
180 (170–380)
pol30-52 160 (120–260)180380
190 (120–210)
5 × 15Wild type3.4 (2.9–7.7)3.01
2.8 (2.4–5.5)
msh2 57 (39–98)4916
50 (39–110)
43 (32–55)
pol30-52 59 (45–120)6120
68 (47–110)
8 × 10Wild type0.96 (0.72–1.2)0.941
1.1 (0.74–2.0)
msh2 14 (9.2–22)1112
11 (4.7–22)
pol30-52 62 (38–140)6265
62 (40–87)
20 × 3Wild type6.9 (5.4–11)7.41
7.8 (6.5–10)
msh2 5.5 (4.4–11)6.91
15 (11–19)
12 (10–18)
pol30-52 57 (45–69)436
29 (19–53)

Data on the rates of instability in wild-type and msh2 strains were from Sia et al. (1997b) and Kokoska et al. (1998).

  • a The first number is the number of base pairs per repeat unit, and the second is the number of repeats within the plasmid-borne tract.

  • b All strains are isogenic except for the indicated mutation.

  • c Numbers outside of parentheses represent rates calculated by the method of the median based on measuring the frequency of 5-FOAR cells in 10–20 independant cultures. Numbers in parentheses are 95% confidence limits.

  • d The rates of 5-FOAR derivatives were multiplied by the proportion of those derivatives in which the repetitive tract had a size alteration (always >0.8) to yield the rates of tract alterations. These rates were averaged for each strain.