TABLE 4

Effect of rfa1-D228Y on the efficiency of DSB-induced deletion events

Relevant genotypea% Urab% Leu+c
Wild type72 ± 622± 4
rfa1-D228Y 73 ± 518 ± 3
rad1 34 ± 919 ± 6
rad1 rfa1-D228Y 35 ± 720 ± 2
rad52 3 ± 119 ± 4
rad52 rfa1-D228Y 21 ± 418 ± 8
rad1 rad52 0.1 ± 0.05ND
rad1 rad52 rfa1-D228Y 8 ± 115 ± 6
  • a Complete genotypes of the strains used are listed in Table 1.

  • b A comparison of the number of Ura cells present 60 min after HO induction with the number of Trp+ cells present at the zero time point. Percentages are given as the mean ± SD of three to six trials.

  • c The percentage of Ura cells present after HO induction that also display a Leu+ phenotype. Percentages are given as the mean ± SD of three to six trials.