TABLE 3

Distribution of Leu+ recombinants in the distal configuration

DeletionsReplacementsTriplications
GenotypeaEventsbRatecEventsbRatecEventsbRatecLeu+ rated
Wild type3 (2)0.0390 (54)1.07 (4)0.081.1 ± 0.3
rfa1-D228Y 26 (15)0.953 (31)1.921 (12)0.83.6 ± 1.3
rad1 2 (1)0.0181 (46)0.417 (10)0.090.5 ± 0.1
rad1 rfa1-D228Y 2 (1)0.0476 (45)1.422 (13)0.41.8 ± 0.7
rad52 15 (9)0.00517 (10)0.00568 (41)0.020.03 ± 0.01
rad52 rfa1-D228Y 61 (34)0.069 (5)0.00930 (17)0.030.1 ± 0.06
rad1 rad52 0<0.000159 (28)0.00541 (20)0.0030.008 ± 0.003
rad1 rad52 rfa1-D228Y 19 (10)0.0255 (29)0.0626 (14)0.030.1 ± 0.05
  • a The complete genotypes of the strains are listed in Table 1.

  • b Percentage of Leu+ prototrophs for each genotype. The number within parentheses represents the actual number of Leu+ prototrophs detected.

  • c The rate (× 10–5) is determined from the Leu+ rate multiplied by the percent of events in each class.

  • d Rates of total Leu+ recombination (× 10–5) are given as the mean ± SD (Jinks-Robertson and Petes 1986).