TABLE 1

S. cerevisiae strains used in this study

StrainaGenotype
W303-1B MATα ade2-1 can1-100 his3-11,15 leu2-3,112 rad5 trp1-1 ur33-1 rad5-G535R
W1088-1AMATa his5Δ
W1088-10DMATα his5Δ lys2Δ
W1479-11CbMAT::HIS3 RAD5 leu2ΔEcoRI::URA3-HOcs::leu2ΔBstEII
W1479-30CbMAT::HIS3 lys2ΔSpeI RAD5 leu2ΔEcoRI::URA3-HOcs::leu2ΔBstEII rfa1-D228Y
W1479-68CbMAT::HIS3 lys2ΔSpeI RAD5 leu2ΔEcoRI::URA3-HOcs::leu2ΔBstEII rad1::HIS5
W1479-18CbMAT::HIS3 RAD5 leu2ΔEcoRI::URA3-HOcs::leu2ΔBstEII rad1::HIS5 rfa1-D228Y
W1479-29AbMAT::HIS3 lys2ΔSpeI RAD5 leu2ΔEcoRI::URA3-HOcs::leu2ΔBstEII rad52::HIS5
W1479-80BbMAT::HIS3 lys2ΔSpeI RAD5 leu2ΔEcoRI::URA3-HOcs::leu2ΔBstEII rad52::HIS5 rfa1-D228Y
W1479-82AbMAT::HIS3 RAD5 leu2ΔEcoRI::URA3-HOcs::leu2ΔBstEII rad1::HIS5 rad52::HIS5
W1479-53DbMAT::HIS3 lys2ΔSpeI RAD5 leu2ΔEcoRI::URA3-HOcs::leu2ΔBstEII rad1::HIS5 rad52::HIS5 rfa1-D228Y
W1490-16AbMAT::HIS3 RAD5 leu2ΔBstEII::URA3-HOcs::leu2ΔEcoRI
W1490-67DbMAT::HIS3 RAD5 leu2ΔBstEII::URA3-HOcs::leu2ΔEcoRI rfa1-D228Y
W1490-82DbMAT::HIS3 RAD5 leu2ΔBstEII::URA3-HOcs::leu2ΔEcoRI rad1::HIS5
W1490-40BbMAT::HIS3 RAD5 leu2ΔBstEII::URA3-HOcs::leu2ΔEcoRI rad1::HIS5 rfa1-D228Y
W1490-83AbMAT::HIS3 RAD5 leu2ΔBstEII::URA3-HOcs::leu2ΔEcoRI rad52::HIS5
W1490-25CbMAT::HIS3 RAD5 leu2ΔBstEII::URA3-HOcs::leu2ΔEcoRI rad52::HIS5 rfa1-D228Y
W1490-79DbMAT::HIS3 RAD5 leu2ΔBstEII::URA3-HOcs::leu2ΔEcoRI rad1::HIS5 rad52::HIS5
W1490-241CbMAT::HIS3 RAD5 leu2ΔBstEII::URA3-HOcs::leu2ΔEcoRI rad1::HIS5 rad52::HIS5 rfa1-D228Y
  • a All strains used are derivatives of W303-1B (Thomas and Rothstein 1989a), and only genotypic differences are noted.

  • b Several strains with the same genotype were used; however, only one is noted.