TABLE 1

N. crassa strains

StrainRelevant genotypeCommentsSource
74AWild-type AR. L. Weiss (UCLA)
FGSCa 6103 his-3 A J. J. Loros (Dartmouth)
FGSC 4564 ab-3B cyh-1 am1 FGSC
6103/4564his-3 A+ ad-3B cyh-1 am1 heterokaryonThis study
3-7#5 Δ gna-1::mtr+ pdx-1 a Ivey et al. (1996)
30-1 Δ gna-1::mtr+ his-3 pdx-1 a 6103 × 3-7#5 progenybThis study
R-10-2 Δ gna-1::mtr+ his-3+ pdx-1 a Δ gna-1 This study
R-5-7 Δ gna-1::mtr+ his-3+ pdx-1 a Δ gna-1 This study
17-7-7 Δ gna-1::mtr+ gna-1+ ::his-3+ pdx-1 a wild-type gna-1This study
17-3 Δ gna-1::mtr+ gna-1+ ::his-3+ pdx-1 a wild-type gna-1This study
15-9-2 Δ gna-1::mtr+ gna-1 R178C ::his-3+ pdx-1 a gna-1R178C alleleThis study
15-38 Δ gna-1::mtr+ gna-1 R178C ::his-3+ pdx-1 a gna-1R178C alleleThis study
21-5-1 Δ gna-1::mtr+ gna-1Q 204L ::his-3+ pdx-1 a gna-1Q204L alleleThis study
21-3-7 Δ gna-1::mtr+ gna-1Q 204L ::his-3+ pdx-1 a gna-1Q204L alleleThis study
  • a FGSC: Fungal Genetics Stock Center, Kansas City.

  • b The his-36103 strain was crossed as a heterokaryon with the am1 4564 helper strain to strain 3-7#5. Because the am1 nucleus is not passed during crosses, progeny carry genes from 6103 and 3-7#5 only.